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  • Featured Product
  • KD/KO Validated

DRG2 Monoclonal antibody

DRG2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3A3E1

Cat no : 67164-1-Ig

Synonyms

DRG 2, DRG2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DRG2 Monoclonal antibody (67164-1-Ig)

    WB analysis using 67164-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67164-1-Ig (DRG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DRG2 Monoclonal antibody (67164-1-Ig)

    IF Staining of U2OS using 67164-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using DRG2 antibody (67164-1-Ig, Clone: 3A3E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of Saos-2 cells using DRG2 Monoclonal antibody (67164-1-Ig)

    IF Staining of Saos-2 using 67164-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using DRG2 antibody (67164-1-Ig, Clone: 3A3E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, SMMC-7721 cells, HSC-T6 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inU2OS cells, Saos-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67164-1-Ig targets DRG2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DRG2 fusion protein Ag6878 相同性解析による交差性が予測される生物種
Full Name developmentally regulated GTP binding protein 2
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC000493
Gene symbol DRG2
Gene ID (NCBI) 1819
RRIDAB_2882460
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DRG2 antibody 67164-1-IgDownload protocol
IF protocol for DRG2 antibody 67164-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Int J Mol Sci

PMID: 31861806

Authors - Hye Ryeong Lim
  • KO Validated
View Article