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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DRG2 Monoclonal antibody

DRG2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A3E1

Cat no : CL488-67164

Synonyms

TRAFAC GTPase DRG2, EC:3.6.5.-, DRG-2, DRG 2, Developmentally-regulated GTP-binding protein 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated DRG2 Monoclonal anti (CL488-67164)

    IF Staining of U2OS using CL488-67164

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 DRG2 antibody (CL488-67164, Clone: 3A3E1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated DRG2 Monoclonal anti (CL488-67164)

    FC experiment of U2OS using CL488-67164

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated DRG2 Monoclonal antibody (CL488-67164, Clone:3A3E1)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67164 targets DRG2 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DRG2 fusion protein Ag6878 相同性解析による交差性が予測される生物種
Full Name developmentally regulated GTP binding protein 2
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC000493
Gene symbol DRG2
Gene ID (NCBI) 1819
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DRG2 antibody CL488-67164Download protocol
FC protocol for CL Plus 488 DRG2 antibody CL488-67164Download protocol
Standard Protocols
Click here to view our Standard Protocols