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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUSP22 Monoclonal antibody (68032-1-Ig)

    WB analysis using 68032-1-Ig (same clone as 68032-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68032-1-Ig (DUSP22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68032-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit brain tissue using DUSP22 Monoclonal antibody (68032-1-Ig)

    WB analysis of rabbit brain using 68032-1-Ig (same clone as 68032-1-PBS)

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68032-1-Ig (DUSP22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68032-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using DUSP22 Monoclonal antibody (68032-1-Ig)

    IF Staining of NIH/3T3 using 68032-1-Ig (same clone as 68032-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using DUSP22 antibody (68032-1-Ig, Clone: 3B8F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68032-1-PBS in a different storage buffer formulation.

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DUSP22 Monoclonal antibody, PBS Only

DUSP22 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68032-1-PBS
Clone No.3B8F1

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DUSP22, FLJ35864, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68032-1-PBS targets DUSP22 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DUSP22 fusion protein Ag9831 相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 22
Calculated molecular weight 184 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC022847
Gene Symbol DUSP22
Gene ID (NCBI) 56940
RRIDAB_2918774
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRW4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DUSP22(Dual specificity protein phosphatase 22) is also named as JSP1, LMWDSP2, MKPX. It has been demonstrated to belong to a new subgroup of small DSPs anchored at the membranes by an N-terminal myristic acid moiety and DUSP22 play a regulatory role in the JNK or p38 MAPK pathways(PMID:17384676). It has 2 isoforms produced by alternative splicing with the molecular weight of 21 kDa and 23 kDa.