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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DUSP22 Monoclonal antibody

DUSP22 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B8F1

Cat no : 68032-1-Ig

Synonyms

DUSP22, FLJ35864, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUSP22 Monoclonal antibody (68032-1-Ig)

    WB analysis using 68032-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68032-1-Ig (DUSP22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit brain tissue using DUSP22 Monoclonal antibody (68032-1-Ig)

    WB analysis of rabbit brain using 68032-1-Ig

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68032-1-Ig (DUSP22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using DUSP22 Monoclonal antibody (68032-1-Ig)

    IF Staining of NIH/3T3 using 68032-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using DUSP22 antibody (68032-1-Ig, Clone: 3B8F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inrat brain tissue, rabbit brain tissue, mouse brain tissue
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68032-1-Ig targets DUSP22 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DUSP22 fusion protein Ag9831 相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 22
Calculated molecular weight 184 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC022847
Gene symbol DUSP22
Gene ID (NCBI) 56940
RRIDAB_2918774
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DUSP22(Dual specificity protein phosphatase 22) is also named as JSP1, LMWDSP2, MKPX. It has been demonstrated to belong to a new subgroup of small DSPs anchored at the membranes by an N-terminal myristic acid moiety and DUSP22 play a regulatory role in the JNK or p38 MAPK pathways(PMID:17384676). It has 2 isoforms produced by alternative splicing with the molecular weight of 21 kDa and 23 kDa.

Protocols

Product Specific Protocols
WB protocol for DUSP22 antibody 68032-1-IgDownload protocol
IF protocol for DUSP22 antibody 68032-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols