Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68032 targets DUSP22 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.
| Tested Reactivity | Human, Mouse, Rat, Rabbit |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | DUSP22 fusion protein Ag9831 相同性解析による交差性が予測される生物種 |
| Full Name | dual specificity phosphatase 22 |
| Calculated molecular weight | 184 aa, 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC022847 |
| Gene Symbol | DUSP22 |
| Gene ID (NCBI) | 56940 |
| RRID | AB_3084391 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NRW4 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DUSP22(Dual specificity protein phosphatase 22) is also named as JSP1, LMWDSP2, MKPX. It has been demonstrated to belong to a new subgroup of small DSPs anchored at the membranes by an N-terminal myristic acid moiety and DUSP22 play a regulatory role in the JNK or p38 MAPK pathways(PMID:17384676). It has 2 isoforms produced by alternative splicing with the molecular weight of 21 kDa and 23 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DUSP22 antibody CL488-68032 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |