Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 13740-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 13740-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 13740-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13740-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13740-1-AP (DUT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 13740-1-AP
IP result of anti-DUT (IP:13740-1-AP, 4ug; Detection:13740-1-AP 1:800) with HEK-293 cells lysate 1440 ug.
IHC staining of human heart using 13740-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13740-1-AP (DUT Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 13740-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13740-1-AP (DUT Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 13740-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DUT antibody (13740-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

DUT Polyclonal antibody

DUT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13740-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EC:3.6.1.23, dUTPase, deoxyuridine triphosphatase, Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human liver tissue, human kidney tissue, HEK-293 cells, Ramos cells, A549 cells, HepG2 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
CoIP	See 1 publications below

Product Information

13740-1-AP targets DUT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DUT fusion protein Ag4677 相同性解析による交差性が予測される生物種
Full Name	deoxyuridine triphosphatase
Calculated molecular weight	252 aa, 27 kDa
Observed molecular weight	23 kDa
GenBank accession number	BC033645
Gene Symbol	DUT
Gene ID (NCBI)	1854
RRID	AB_2093170
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P33316
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for DUT antibody 13740-1-AP	Download protocol
IHC protocol for DUT antibody 13740-1-AP	Download protocol
IF protocol for DUT antibody 13740-1-AP	Download protocol
IP protocol for DUT antibody 13740-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP	Cell Metab Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis. Authors - Yi-Ping Wang View Article
human	WB	Cell Chem Biol CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity. Authors - Ying Yu Liang View Article
human	WB	Bioconjug Chem ATP-Linked Chimeric Nucleotide as a Specific Luminescence Reporter of Deoxyuridine Triphosphatase. Authors - Debin Ji View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13740-1-AP

WB analysis of human liver using 13740-1-AP

WB analysis of human kidney using 13740-1-AP

WB analysis of HEK-293 using 13740-1-AP

IP experiment of HEK-293 using 13740-1-AP

IHC staining of human heart using 13740-1-AP