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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated DUT Polyclonal antibody

DUT Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13740

Synonyms

EC:3.6.1.23, dUTPase, deoxyuridine triphosphatase, Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated DUT Polyclonal antib (CL488-13740)

    IF Staining of HeLa using CL488-13740

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 DUT antibody (CL488-13740) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated DUT Polyclonal antib (CL488-13740)

    FC experiment of U2OS using CL488-13740

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 DUT Polyclonal Antibody (CL488-13740)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13740 targets DUT in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUT fusion protein Ag4677 相同性解析による交差性が予測される生物種
Full Name deoxyuridine triphosphatase
Calculated molecular weight 252 aa, 27 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC033645
Gene symbol DUT
Gene ID (NCBI) 1854
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DUT antibody CL488-13740Download protocol
Standard Protocols
Click here to view our Standard Protocols