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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DVL2 Monoclonal antibody

DVL2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2F2D2

Cat no : 67105-1-Ig

Synonyms

Dishevelled 2, DSH homolog 2, DVL2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DVL2 Monoclonal antibody (67105-1-Ig)

    WB analysis using 67105-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DVL2 Monoclonal antibody (67105-1-Ig)

    WB analysis using 67105-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using DVL2 Monoclonal antibody (67105-1-Ig)

    WB analysis of T-47D using 67105-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using DVL2 Monoclonal antibody (67105-1-Ig)

    WB analysis of A431 using 67105-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using DVL2 Monoclonal antibody (67105-1-Ig)

    IHC staining of mouse colon using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using DVL2 Monoclonal antibody (67105-1-Ig)

    IHC staining of mouse colon using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using DVL2 Monoclonal antibody (67105-1-Ig)

    IHC staining of rat colon using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using DVL2 Monoclonal antibody (67105-1-Ig)

    IHC staining of rat colon using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using DVL2 Monoclonal antibody (67105-1-Ig)

    IHC staining of human gliomas using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using DVL2 Monoclonal antibody (67105-1-Ig)

    IHC staining of human gliomas using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DVL2 Monoclonal antibody (67105-1-Ig)

    IHC staining of human colon cancer using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DVL2 Monoclonal antibody (67105-1-Ig)

    IHC staining of human colon cancer using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DVL2 Monoclonal antibody (67105-1-Ig)

    IHC staining of human prostate cancer using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DVL2 Monoclonal antibody (67105-1-Ig)

    IHC staining of human prostate cancer using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inMCF-7 cells, A431 cells, T-47D cells, THP-1 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse colon tissue, human colon cancer tissue, human gliomas tissue, human prostate cancer tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67105-1-Ig targets DVL2 in WB, IHC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DVL2 fusion protein Ag28634 相同性解析による交差性が予測される生物種
Full Name dishevelled, dsh homolog 2 (Drosophila)
Calculated molecular weight 736 aa, 79 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC014844
Gene symbol DVL2
Gene ID (NCBI) 1856
RRIDAB_2882409
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DVL2 antibody 67105-1-IgDownload protocol
IHC protocol for DVL2 antibody 67105-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Int J Biol Sci

CYR61 Acts as an Intracellular Microtubule-Associated Protein and Coordinates Mitotic Progression via PLK1-FBW7 Pathway

Authors - Kaishun Hu
View Article
ratWB

Biochim Biophys Acta Mol Cell Res

Wnt/β-catenin signaling inhibits oxidative stress-induced ferroptosis to improve interstitial cystitis/bladder pain syndrome by reducing NF-κB

Authors - Weilin Fang
View Article