Validation Data Gallery

Filter: AllWBIHC

WB analysis using 67105-1-Ig (same clone as 67105-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 67105-1-Ig (same clone as 67105-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.
WB analysis using 67105-1-Ig (same clone as 67105-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.
WB analysis of T-47D using 67105-1-Ig (same clone as 67105-1-PBS)
T-47D cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 67105-1-Ig (same clone as 67105-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 67105-1-Ig (same clone as 67105-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 67105-1-Ig (same clone as 67105-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 67105-1-Ig (same clone as 67105-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67105-1-Ig (same clone as 67105-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 67105-1-Ig (same clone as 67105-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 67105-1-Ig (same clone as 67105-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67105-1-Ig (same clone as 67105-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 67105-1-Ig (same clone as 67105-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 67105-1-Ig (same clone as 67105-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67105-1-PBS in a different storage buffer formulation.

DVL2 Monoclonal antibody, PBS Only

DVL2 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67105-1-PBS

Clone No.2F2D2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Dishevelled 2, 2F2D2, Dishevelled-2, DSH homolog 2, Segment polarity protein dishevelled homolog DVL-2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67105-1-PBS targets DVL2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag28634 Product name: Recombinant human DVL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-340 aa of BC014844 Sequence: MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDI 相同性解析による交差性が予測される生物種
Full Name	dishevelled, dsh homolog 2 (Drosophila)
Calculated molecular weight	736 aa, 79 kDa
Observed molecular weight	90 kDa
GenBank accession number	BC014844
Gene Symbol	DVL2
Gene ID (NCBI)	1856
RRID	AB_2882409
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	O14641
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 67105-1-Ig (same clone as 67105-1-PBS)

WB analysis of A431 using 67105-1-Ig (same clone as 67105-1-PBS)

WB analysis using 67105-1-Ig (same clone as 67105-1-PBS)

WB analysis of T-47D using 67105-1-Ig (same clone as 67105-1-PBS)

IHC staining of mouse colon using 67105-1-Ig (same clone as 67105-1-PBS)