ChromoTek DYKDDDDK Fab-Trap® Agarose Kit
The ChromoTek DYKDDDDK Fab-Trap® Agarose Kit consists of an anti-DYKDDDDK-tag Fab fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap® Agarose Kit is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc
Specificity
Flag®-tag (1x and 3x)
Applications
IP, CoIP, ChIP, RIP, Protein purification
Type
Fab-fragment
Conjugate
Agarose
Cat no : ffak
Synonyms
Validation Data Gallery
Product Information
The ChromoTek DYKDDDDK Fab-Trap® Agarose Kit consists of an anti-DYKDDDDK-tag Fab fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap® Agarose Kit is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc
Description | The DYKDDDDK Fab-Trap Agarose Kit contains DYKDDDDK Fab-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of Flag®-tagged proteins and their interacting factors. • Lowest background in class • Less contaminating antibody chains • Recombinantly expressed Fab-fragment derived • Compatible with harsh buffer conditions • Ready-to-use |
Applications | IP, CoIP, ChIP, RIP, Protein purification |
Specificity/Target | The DYKDDDDK Fab-Trap® Agarose specifically binds to 1x and 3xDYKDDDDK-tag sequence (1x and 3xFlag®-tag). It binds to same epitope as Sigma's Anti-FLAG® Antibody and is compatible with N-, C-terminal or internal tagging. |
Binding capacity | 12.5 - 15 µg of recombinant 3xDYKDDDDK-tagged protein (~30 kDa) per 25µL bead slurry |
Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
Elution buffer | 3xDYKDDDDK-peptide SDS sample buffer 0.2 M glycine pH 2.5 |
Wash buffer compatibility | 0.5 % Nonidet P40 Substitute, 5 % Tween™ 20, 2 M NaCl, 10 mM TCEP, 10 mM DTT, 10 mM ß-mercaptoethanol, 2 M Urea |
Type | Fab-fragment |
Class | Recombinant |
Host | Mouse |
RRID | AB_2894836 |
Storage Buffer | 1x PBS, Preservative: 0.09 % sodium azide |
Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
Kit components
Component | Description |
---|---|
Dilution buffer | Dilution of cell lysate |
Elution buffer | For acidic elution |
Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
Wash buffer | Removal of unwanted proteins, peptides, etc. |
DYKDDDDK Fab-Trap™ Agarose | 20 reactions (500 µL slurry) |
Documentation
SDS |
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SDS ffak-DYKDDDDK Fab Trap Agarose Kit (PDF) |
Protocol |
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Protocol DYKDDDDK Fab-Trap® Agarose Kit (PDF) |
Publications
Application | Title |
---|---|
Nat Commun A spatio-temporally constrained gene regulatory network directed by PBX1/2 acquires limb patterning specificity via HAND2 | |
Sci Adv Nuclear pyruvate dehydrogenase complex regulates histone acetylation and transcriptional regulation in the ethylene response | |