Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | Transfected HEK-293T cells |
The antibody 80010-1-RR is suggested for IP and IF.
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-80010 targets DYKDDDDK tag in IF/ICC applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2329 Product name: Recombinant DYKDDDDK tag protein Source: e coli.-derived, PGEX-4T Tag: GST Sequence: DYKDDDDK 相同性解析による交差性が予測される生物種 |
| Full Name | DYKDDDDK tag |
| Gene Symbol | |
| Gene ID (NCBI) | |
| Conjugate | CoraLite® Plus 555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 554 nm / 570 nm |
| 激发激光 | Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | FLAGTAG |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 555 DYKDDDDK tag antibody CL555-80010 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |