CoraLite® Plus 750-conjugated DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Polyclonal Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

Cat no : CL750-20543

Synonyms

DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag


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Tested Applications

Positive WB detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-20543 targets DYKDDDDK tag in WB applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYKDDDDK tag fusion protein Ag2329 相同性解析による交差性が予測される生物種
Full Name DYKDDDDK tag
Gene symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 DYKDDDDK tag antibody CL750-20543Download protocol
Standard Protocols
Click here to view our Standard Protocols