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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated DYNC1H1 Polyclonal a (CL488-12345)

    IF Staining of MCF-7 using CL488-12345

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 DYNC1H1 antibody (CL488-12345) at dilution of 1:200.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DYNC1H1 Polyclonal antibody

DYNC1H1 Polyclonal Antibody for IF/ICC
Cat No. CL488-12345

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, zebrafish, mouse

Applications

IF/ICC

Synonyms

DHC1, DHC1a, DKFZp686P2245, DNCH1, Dnchc1, DNCL, DNECL, DYHC, DYNC1H1, Dynein heavy chain, cytosolic, HL 3, KIAA0325, p22

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12345 targets DYNC1H1 in IF/ICC applications and shows reactivity with human, rat, zebrafish, mouse samples.

Tested Reactivity human, rat, zebrafish, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYNC1H1 fusion protein Ag2999 相同性解析による交差性が予測される生物種
Full Name dynein, cytoplasmic 1, heavy chain 1
Calculated molecular weight 4646 aa, 532 kDa
Observed molecular weight 532 kDa
GenBank accession numberBC021297
Gene Symbol DYNC1H1
Gene ID (NCBI) 1778
RRIDAB_3083925
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14204
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dyneins are a group of microtubule-activated ATPases that serve to convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients..

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DYNC1H1 antibody CL488-12345Download protocol
Standard Protocols
Click here to view our Standard Protocols