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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using DYNC2H1 Polyclonal antibody (29758-1-AP)

    WB analysis of mouse testis using 29758-1-AP (same clone as 29758-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 29758-1-AP (DYNC2H1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using DYNC2H1 Polyclonal antibody (29758-1-AP)

    IHC staining of mouse kidney using 29758-1-AP (same clone as 29758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29758-1-AP (DYNC2H1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using DYNC2H1 Polyclonal antibody (29758-1-AP)

    IHC staining of mouse kidney using 29758-1-AP (same clone as 29758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29758-1-AP (DYNC2H1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29758-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using DYNC2H1 Polyclonal antibody (29758-1-AP)

    IF Staining of hTERT-RPE1 using 29758-1-AP (same clone as 29758-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DYNC2H1 antibody (29758-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green). This data was developed using the same antibody clone with 29758-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using DYNC2H1 Polyclonal antibody (29758-1-AP)

    IF Staining of hTERT-RPE1 using 29758-1-AP (same clone as 29758-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using DYNC2H1 antibody (29758-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green). This data was developed using the same antibody clone with 29758-1-PBS in a different storage buffer formulation.

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DYNC2H1 Polyclonal antibody, PBS Only

DYNC2H1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29758-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29758-1-PBS targets DYNC2H1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYNC2H1 fusion protein Ag30707 相同性解析による交差性が予測される生物種
Full Name dynein, cytoplasmic 2, heavy chain 1
Calculated molecular weight 493 kDa
Observed molecular weight493 kDa
GenBank accession numberNM_001377
Gene Symbol DYNC2H1
Gene ID (NCBI) 79659
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NCM8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DYNC2H1 (Cytoplasmic dynein 2 heavy chain 1) is involved in ciliary intraflagellar transport (IFT). DYNC2H1 drives retrograde transport of the IFT-A protein complex that regulates tip-to-base transport in cilia, involved in the generation and maintenance of cilia. DYNC2H1 variants or retina-predominant variants cause nonsyndromic retinal degeneration (PMID:32753734). DYNC2H1 mutations also cause asphyxiating thoracic dystrophy and short rib-polydactyly syndrome (PMID: 19442771). DYNC2H1 was localized to the basal bodies by immunofluorescence staining (PMID: 21552265, PMID: 26077881, PMID: 30320547).