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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated DYNLT1 Monoclonal an (CL488-68312)

    IF Staining of HepG2 using CL488-68312

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 DYNLT1 antibody (CL488-68312, Clone: 2E3D4 ) at dilution of 1:200, CL594-Phalloidin (red).

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CoraLite® Plus 488-conjugated DYNLT1 Monoclonal antibody

DYNLT1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68312
Clone No.2E3D4

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

T-complex testis-specific protein 1 homolog, Protein CW-1, Protein CW 1, Dynein light chain Tctex-type 1, CW 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68312 targets DYNLT1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DYNLT1 fusion protein Ag33072 相同性解析による交差性が予測される生物種
Full Name dynein, light chain, Tctex-type 1
Calculated molecular weight 113 aa, 12 kDa
Observed molecular weight12 kDa
GenBank accession numberBC029412
Gene Symbol DYNLT1
Gene ID (NCBI) 6993
RRIDAB_3672993
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP63172
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DYNLT (or Tctex-1) was originally described as a light chain component of the dynein motor complex. Tctex-1 also has several dynein-independent functions, including roles in G protein signaling activation and neuronal growth. Tctex-1 is selectively enriched in proliferating neural progenitors of both embryonic and adult brains. Genetic knockdown of Tctex-1 in radial precursors promoted neurogenesis, indicating its implication in regulation of cortical neurogenesis. (PMID: 19448628)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DYNLT1 antibody CL488-68312Download protocol
Standard Protocols
Click here to view our Standard Protocols