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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated Decorin Monoclonal antibody

Decorin Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3A4E4

Cat no : CL647-66847

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using CoraLite® Plus 647-conjugated Decorin Monoclonal a (CL647-66847)

    IF Staining of BxPC-3 using CL647-66847

    Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CoraLite® Plus 647 Decorin antibody (CL647-66847, Clone: 3A4E4 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66847 targets Decorin in IF/ICC applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Decorin fusion protein Ag6275 相同性解析による交差性が予測される生物種
Full Name decorin
Calculated molecular weight 359 aa, 40 kDa
Observed molecular weight 40 kDa, 100 kDa
GenBank accession numberBC005322
Gene symbol Decorin
Gene ID (NCBI) 1634
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Decorin antibody CL647-66847Download protocol
Standard Protocols
Click here to view our Standard Protocols