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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Drebrin Monoclonal antibody, PBS Only

Drebrin Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A12H1

Cat no : 67589-1-PBS

Synonyms

D0S117E, DBN1, DKFZp434D064, Drebrin, drebrin 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Drebrin Monoclonal antibody (67589-1-Ig)

    WB analysis using 67589-1-Ig (same clone as 67589-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67589-1-Ig (Drebrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67589-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Drebrin Monoclonal antibody (67589-1-Ig)

    WB analysis using 67589-1-Ig (same clone as 67589-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67589-1-Ig (Drebrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67589-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Drebrin Monoclonal antibody (67589-1-Ig)

    IHC staining of mouse brain using 67589-1-Ig (same clone as 67589-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67589-1-Ig (Drebrin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67589-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Drebrin Monoclonal antibody (67589-1-Ig)

    IF Staining of mouse brain using 67589-1-Ig (same clone as 67589-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Drebrin antibody (67589-1-Ig, Clone: 1A12H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67589-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67589-1-PBS targets Drebrin in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Drebrin fusion protein Ag22635 相同性解析による交差性が予測される生物種
Full Name drebrin 1
Observed molecular weight 110-120 kDa
GenBank accession numberBC007567
Gene symbol DBN1
Gene ID (NCBI) 1627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.