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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Drebrin Monoclonal antibody

Drebrin Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A12H1

Cat no : 67589-1-Ig

Synonyms

D0S117E, DBN1, DKFZp434D064, Drebrin, drebrin 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Drebrin Monoclonal antibody (67589-1-Ig)

    WB analysis using 67589-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67589-1-Ig (Drebrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Drebrin Monoclonal antibody (67589-1-Ig)

    WB analysis using 67589-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67589-1-Ig (Drebrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Drebrin Monoclonal antibody (67589-1-Ig)

    IHC staining of mouse brain using 67589-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67589-1-Ig (Drebrin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Drebrin Monoclonal antibody (67589-1-Ig)

    IF Staining of mouse brain using 67589-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Drebrin antibody (67589-1-Ig, Clone: 1A12H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue).

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Tested Applications

Positive WB detected inHeLa cells, pig brain tissue, HEK-293 cells, PC-12 cells, Neuro-2a cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67589-1-Ig targets Drebrin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Drebrin fusion protein Ag22635 相同性解析による交差性が予測される生物種
Full Name drebrin 1
Observed molecular weight 110-120 kDa
GenBank accession numberBC007567
Gene symbol Drebrin
Gene ID (NCBI) 1627
RRIDAB_2882797
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

Protocols

Product Specific Protocols
WB protocol for Drebrin antibody 67589-1-IgDownload protocol
IHC protocol for Drebrin antibody 67589-1-IgDownload protocol
IF protocol for Drebrin antibody 67589-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols