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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Drebrin Monoclonal antibody

Drebrin Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A12H1

Cat no : CL594-67589

Synonyms

DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 1A12H1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated Drebrin Monoclonal antibod (CL594-67589)

    IF Staining of mouse brain using CL594-67589

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Drebrin antibody (CL594-67589, Clone: 1A12H1 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite®594-conjugated Drebrin Monoclonal antibod (CL594-67589)

    IF Staining of SH-SY5Y using CL594-67589

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®594 Drebrin antibody (CL594-67589, Clone: 1A12H1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite®594-conjugated Drebrin Monoclonal antibod (CL594-67589)

    FC experiment of SH-SY5Y using CL594-67589

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Drebrin (CL594-67589, Clone:1A12H1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67589 targets Drebrin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Drebrin fusion protein Ag22635 相同性解析による交差性が予測される生物種
Full Name drebrin 1
Observed molecular weight 110-120 kDa
GenBank accession numberBC007567
Gene symbol Drebrin
Gene ID (NCBI) 1627
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

Protocols

Product Specific Protocols
IF protocol for CL594 Drebrin antibody CL594-67589Download protocol
Standard Protocols
Click here to view our Standard Protocols