CoraLite® Plus 488-conjugated Dystrophin Monoclonal antibody
Dystrophin Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
IF/ICC, IF-P, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1G12E6
Cat no : CL488-68120
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse skeletal muscle tissue |
| Positive IF/ICC detected in | H9C2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68120 targets Dystrophin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Dystrophin fusion protein Ag4392 相同性解析による交差性が予測される生物種 |
| Full Name | dystrophin |
| Calculated molecular weight | 3685 aa, 427 kDa |
| Observed molecular weight | 70 kDa, 430 kDa |
| GenBank accession number | BC028720 |
| Gene symbol | Dystrophin |
| Gene ID (NCBI) | 1756 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Dystrophin antibody CL488-68120 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
