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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

E2F4 Monoclonal antibody

E2F4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2D1E1

Cat no : 67812-1-Ig

Synonyms

E2F 4, E2F4, Transcription factor E2F4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using E2F4 Monoclonal antibody (67812-1-Ig)

    WB analysis using 67812-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of various lysates using E2F4 Monoclonal antibody (67812-1-Ig)

    WB analysis using 67812-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67812-1-Ig targets E2F4 in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen E2F4 fusion protein Ag28533 相同性解析による交差性が予測される生物種
Full Name E2F transcription factor 4, p107/p130-binding
Calculated molecular weight 44 kDa
GenBank accession numberBC033180
Gene symbol E2F4
Gene ID (NCBI) 1874
RRIDAB_2918575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.

Protocols

Product Specific Protocols
WB protocol for E2F4 antibody 67812-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Leukemia

In vivo CRISPR/Cas9-mediated screen reveals a critical function of TFDP1 and E2F4 transcription factors in hematopoiesis

Authors - Ngoc Tung Tran
View Article
humanWB,IF,CoIP

Int J Oncol

Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9

Authors - Zhenwei Ma
  • KD Validated
View Article