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  • Featured Product
  • KD/KO Validated

E2F4 Monoclonal antibody, PBS Only

E2F4 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D1E1

Cat no : 67812-1-PBS

Synonyms

Transcription factor E2F4, E2F-4, E2F 4, 2D1E1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using E2F4 Monoclonal antibody (67812-1-Ig)

    WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67812-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using E2F4 Monoclonal antibody (67812-1-Ig)

    WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67812-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67812-1-PBS targets E2F4 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen E2F4 fusion protein Ag28533 相同性解析による交差性が予測される生物種
Full Name E2F transcription factor 4, p107/p130-binding
Calculated molecular weight 44 kDa
GenBank accession numberBC033180
Gene symbol E2F4
Gene ID (NCBI) 1874
RRIDAB_2918575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.