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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ECHS1 Polyclonal antibody

ECHS1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11305

Synonyms

ECHS1, Enoyl CoA hydratase 1, SCEH

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated ECHS1 Polyclonal ant (CL488-11305)

    IF Staining of HeLa using CL488-11305

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 ECHS1 antibody (CL488-11305) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated ECHS1 Polyclonal ant (CL488-11305)

    IF Staining of HeLa using CL488-11305

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 ECHS1 antibody (CL488-11305) at dilution of 1:1600.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11305 targets ECHS1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECHS1 fusion protein Ag1842 相同性解析による交差性が予測される生物種
Full Name enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Calculated molecular weight 31 kDa
Observed molecular weight 29-31 kDa
GenBank accession numberBC008906
Gene symbol ECHS1
Gene ID (NCBI) 1892
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ECHS1 antibody CL488-11305Download protocol
Standard Protocols
Click here to view our Standard Protocols