CoraLite® Plus 488-conjugated ECSIT Recombinant antibody
ECSIT Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240223G2
Cat no : CL488-83295-5
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of CL488-83295-5 is ECSIT Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ECSIT fusion protein Ag26898 相同性解析による交差性が予測される生物種 |
| Full Name | ECSIT homolog (Drosophila) |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 49 kDa |
| GenBank accession number | BC008279 |
| Gene symbol | ECSIT |
| Gene ID (NCBI) | 51295 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ECSIT is a cytosolic adaptor protein associated with the toll-like receptor pathway. It has a distinct N-terminal mitochondrial targeting sequence, pentatricopeptide repeat motif, and a C-terminal pleckstrin homology domain. ECSIT regulates many biological processes like embryonic development, inflammation, cardiac function, and assembly of mitochondrial complex I. In macrophages, the activation of TLRs by inflammatory signals promotes the translocation of TRAF6 to mitochondria where it engages ECSIT to stimulate the production of mitochondrial reactive oxygen species (ROS).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ECSIT antibody CL488-83295-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
