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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated EDC4 Polyclonal anti (CL488-17737)

    IF Staining of HeLa using CL488-17737

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 EDC4 antibody (CL488-17737) at dilution of 1:400, CL594-phalloidin (red).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated EDC4 Polyclonal antibody

EDC4 Polyclonal Antibody for IF/ICC
Cat No. CL488-17737

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

Autoantigen Ge 1, Autoantigen RCD 8, EDC4, enhancer of mRNA decapping 4, Ge 1, HEDLS, RCD 8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17737 targets EDC4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EDC4 fusion protein Ag11784 相同性解析による交差性が予測される生物種
Full Name enhancer of mRNA decapping 4
Calculated molecular weight 1401 aa, 152 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC064567
Gene Symbol EDC4
Gene ID (NCBI) 23644
RRIDAB_3084007
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P2E9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 EDC4 antibody CL488-17737Download protocol
Standard Protocols
Click here to view our Standard Protocols