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  • KD/KO Validated

EEA1 Monoclonal antibody

EEA1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E11E4

Cat no : 68065-1-Ig

Synonyms

early endosome antigen 1, EEA1, MST105, MSTP105, ZFYVE2


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Tested Applications

Positive WB detected inA431 cells, HepG2 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68065-1-Ig targets EEA1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEA1 fusion protein Ag28814 相同性解析による交差性が予測される生物種
Full Name early endosome antigen 1
Calculated molecular weight 162 kDa
Observed molecular weight 162 kDa
GenBank accession numberNM_003566
Gene symbol EEA1
Gene ID (NCBI) 8411
RRIDAB_2918806
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

Protocols

Product Specific Protocols
WB protocol for EEA1 antibody 68065-1-IgDownload protocol
IF protocol for EEA1 antibody 68065-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Life Sci Alliance

ACE2-EGFR-MAPK signaling contributes to SARS-CoV-2 infection

Authors - Melanie Engler
humanIF

J Alzheimers Dis

High Fat Diet Mediates Amyloid-β Cleaving Enzyme 1 Phosphorylation and SUMOylation, Enhancing Cognitive Impairment in APP/PS1 Mice

Authors - Jian Bao
mouseIF

Antioxidants (Basel)

Oxybaphus himalaicus Mitigates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting TLR4/MD2 Complex Formation

Authors - Honghong Zhan
humanIF

Cell Chem Biol

Targeting VPS41 induces methuosis and inhibits autophagy in cancer cells

Authors - Yue Liu
humanIF

Am J Physiol Cell Physiol

Progranulin and EGFR modulate receptor-like tyrosine kinase sorting and stability in mesothelioma cells

Authors - Elisa Ventura
humanIF

Cell Death Dis

TRIM65 promotes renal cell carcinoma through ubiquitination and degradation of BTG3

Authors - Qi Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Aditya (Verified Customer) (11-01-2024)

great with ecl

  • Applications: Western Blot