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  • Featured Product
  • KD/KO Validated

EEA1 Monoclonal antibody, PBS Only

EEA1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11E4

Cat no : 68065-1-PBS

Synonyms

ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using EEA1 Monoclonal antibody (68065-1-Ig)

    WB analysis of HepG2 using 68065-1-Ig (same clone as 68065-1-PBS)

    WB result of EEA1 antibody (68065-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEA1 transfected HepG2 cells. This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EEA1 Monoclonal antibody (68065-1-Ig)

    WB analysis using 68065-1-Ig (same clone as 68065-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68065-1-Ig (EEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EEA1 Monoclonal antibody (68065-1-Ig)

    IF Staining of HepG2 using 68065-1-Ig (same clone as 68065-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EEA1 antibody (68065-1-Ig, Clone: 1E11E4 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EEA1 Monoclonal antibody (68065-1-Ig)

    IF Staining of HeLa using 68065-1-Ig (same clone as 68065-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EEA1 antibody (68065-1-Ig, Clone: 1E11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68065-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68065-1-PBS targets EEA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEA1 fusion protein Ag28814 相同性解析による交差性が予測される生物種
Full Name early endosome antigen 1
Calculated molecular weight 162 kDa
Observed molecular weight 162 kDa
GenBank accession numberNM_003566
Gene symbol EEA1
Gene ID (NCBI) 8411
RRIDAB_2918806
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.