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EEA1 Recombinant antibody

EEA1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C11

Cat no : 81245-1-RR

Synonyms

ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using EEA1 Recombinant antibody (81245-1-RR)

    WB analysis of HepG2 using 81245-1-RR

    WB result of EEA1 antibody (81245-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEA1 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using EEA1 Recombinant antibody (81245-1-RR)

    WB analysis using 81245-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81245-1-RR (EEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 81245-1-RR

    Affinity and Kinetic Characterization of 81245-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81245-1-RR against Human EEA1 were performed. The affinity constant is 0.227 nM.

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Tested Applications

Positive WB detected inA431 cells, HepG2 cells, HeLa cells, LNCaP cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81245-1-RR targets EEA1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EEA1 fusion protein Ag28814 相同性解析による交差性が予測される生物種
Full Name early endosome antigen 1
Calculated molecular weight 162 kDa
Observed molecular weight 162 kDa
GenBank accession numberNM_003566
Gene symbol EEA1
Gene ID (NCBI) 8411
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

Protocols

Product Specific Protocols
WB protocol for EEA1 antibody 81245-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols