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CoraLite® Plus 750-conjugated EEA1 Recombinant antibody
EEA1 Recombinant Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
2C11
Cat no : CL750-81245
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HSC-T6 cells, HeLa cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL750-81245 targets EEA1 in WB applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | EEA1 fusion protein Ag28814 相同性解析による交差性が予測される生物種 |
Full Name | early endosome antigen 1 |
Calculated molecular weight | 162 kDa |
Observed molecular weight | 162 kDa |
GenBank accession number | NM_003566 |
Gene symbol | EEA1 |
Gene ID (NCBI) | 8411 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 750 EEA1 antibody CL750-81245 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |