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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EED Monoclonal antibody

EED Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1D11D4

Cat no : 67756-1-Ig

Synonyms

EED, embryonic ectoderm development, HEED, Polycomb protein EED, WAIT 1, WAIT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EED Monoclonal antibody (67756-1-Ig)

    WB analysis using 67756-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67756-1-Ig (EED antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using EED Monoclonal antibody (67756-1-Ig)

    IHC staining of mouse testis using 67756-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67756-1-Ig (EED antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using EED Monoclonal antibody (67756-1-Ig)

    IHC staining of mouse testis using 67756-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67756-1-Ig (EED antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using EED Monoclonal antibody (67756-1-Ig)

    IF Staining of mouse testis using 67756-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EED antibody (67756-1-Ig, Clone: 1D11D4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using EED Monoclonal antibody (67756-1-Ig)

    IF Staining of mouse testis using 67756-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EED antibody (67756-1-Ig, Clone: 1D11D4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inU2OS cells, THP-1 cells, HEK--293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67756-1-Ig targets EED in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EED fusion protein Ag10369 相同性解析による交差性が予測される生物種
Full Name embryonic ectoderm development
Calculated molecular weight 466 aa, 53 kDa
Observed molecular weight50/53/46 kDa.
GenBank accession numberBC068995
Gene symbol EED
Gene ID (NCBI) 8726
RRIDAB_2918524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.

Protocols

Product Specific Protocols
WB protocol for EED antibody 67756-1-IgDownload protocol
IHC protocol for EED antibody 67756-1-IgDownload protocol
IF protocol for EED antibody 67756-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols