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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated EED Monoclonal antibody

EED Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1D11D4

Cat no : CL647-67756

Synonyms

WAIT1, WAIT 1, Polycomb protein EED, Embryonic ectoderm development protein, embryonic ectoderm development

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite® Plus 647-conjugated EED Monoclonal antib (CL647-67756)

    IF Staining of mouse testis using CL647-67756

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 647 EED antibody (CL647-67756, Clone: 1D11D4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite® Plus 647-conjugated EED Monoclonal antib (CL647-67756)

    IF Staining of mouse testis using CL647-67756

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 647 EED antibody (CL647-67756, Clone: 1D11D4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated EED Monoclonal antib (CL647-67756)

    FC experiment of HeLa using CL647-67756

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated EED Monoclonal antibody (CL647-67756, Clone:1D11D4)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG2b isotype control (11B8C4) (CL647-66360-3, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF-P detected inmouse testis tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67756 targets EED in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EED fusion protein Ag10369 相同性解析による交差性が予測される生物種
Full Name embryonic ectoderm development
Calculated molecular weight 466 aa, 53 kDa
GenBank accession numberBC068995
Gene symbol EED
Gene ID (NCBI) 8726
RRIDAB_2920305
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 EED antibody CL647-67756Download protocol
FC protocol for CL Plus 647 EED antibody CL647-67756Download protocol
Standard Protocols
Click here to view our Standard Protocols