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EEF1A1 Polyclonal antibody

Name: EEF1A1 Polyclonal antibody
Brand: Proteintech
SKU: 11402-1-AP
Rating: 5.00 (1 reviews)

EEF1A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 11402-1-AP

Synonyms

eEF1A 1, EEF1A, EEF 1, EC:3.6.5.-, CCS3

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 11402-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11402-1-AP
WB result of EEF1A1 antibody (11402-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1A1 transfected HeLa cells.
WB analysis using 11402-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11402-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 11402-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 11402-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 11402-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11402-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 11402-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 11402-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11402-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11402-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 11402-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 11402-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11402-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 11402-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 11402-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 11402-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF1A1 antibody (11402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 11402-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF1A1 antibody (11402-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 11402-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF1A1 antibody (11402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HeLa using 11402-1-AP
Immunofluorescent analysis of Hela cells, using EEF1A1 antibody 11402-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 11402-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, mouse liver tissue, mouse skeletal muscle tissue, MCF-7 cells, mouse pancreas tissue, mouse ovary tissue, mouse heart tissue, rat brain tissue, human brain tissue, Jurkat cells, SH-SY5Y cells, Neuro-2a cells, C2C12 cells, HepG2 cells, mouse brain tissue
Positive IHC detected in	mouse brain tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 24 publications below
IHC	See 4 publications below
IF	See 7 publications below
IP	See 1 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

11402-1-AP targets EEF1A1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EEF1A1 fusion protein Ag1938 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation elongation factor 1 alpha 1
Calculated molecular weight	462 aa, 50 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC014224
Gene symbol	EEF1A1
Gene ID (NCBI)	1915
RRID	AB_2096966
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell growth and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EEF1A1.

Protocols

Product Specific Protocols
WB protocol for EEF1A1 antibody 11402-1-AP	Download protocol
IHC protocol for EEF1A1 antibody 11402-1-AP	Download protocol
IF protocol for EEF1A1 antibody 11402-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Acetylation of Cytidine in mRNA Promotes Translation Efficiency. Authors - Daniel Arango View Article
mouse	WB	Cell Metab High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate Authors - Peng Zhou View Article
human	WB	Nat Commun Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction Authors - Minji Kim KD Validated View Article
human,mouse	WB,IF	Nat Chem Biol Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis Authors - Yuyin Pan View Article
mouse	WB	Sci Adv CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome. Authors - Sefan Asamitsu View Article
mouse	WB	Dev Cell DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice Authors - Dingfeng Zou View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Tom (Verified Customer) (08-25-2020)

This antibody successfully detects EEF1A1 in western blots. There was one specific and strong band at 50 kDa.

Applications: Western Blot
Cell Tissue Type: Heart

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EEF1A1 Polyclonal antibody

EEF1A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 11402-1-AP

WB analysis of HeLa using 11402-1-AP

WB analysis using 11402-1-AP

WB analysis of mouse liver using 11402-1-AP

WB analysis of mouse pancreas using 11402-1-AP

WB analysis of mouse skeletal muscle using 11402-1-AP

WB analysis of mouse ovary using 11402-1-AP

WB analysis of MCF-7 using 11402-1-AP

WB analysis of mouse heart using 11402-1-AP

WB analysis of rat brain using 11402-1-AP

WB analysis of human brain using 11402-1-AP

WB analysis of Jurkat using 11402-1-AP

WB analysis of SH-SY5Y using 11402-1-AP

IHC staining of mouse brain using 11402-1-AP

IHC staining of mouse brain using 11402-1-AP

IHC staining of human pancreas cancer using 11402-1-AP

IHC staining of human pancreas cancer using 11402-1-AP

IF Staining of HepG2 using 11402-1-AP

IF Staining of HepG2 using 11402-1-AP

IF Staining of HepG2 using 11402-1-AP

IF Staining of HeLa using 11402-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Acetylation of Cytidine in mRNA Promotes Translation Efficiency.

High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate

Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction

Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice

Reviews

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