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  • KD/KO Validated

CoraLite® Plus 488-conjugated EEF1A1 Polyclonal antibody

EEF1A1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11402

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

eEF1A 1, EEF1A, EEF 1, EC:3.6.5.-, CCS3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11402 targets EEF1A1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF1A1 fusion protein Ag1938 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 alpha 1
Calculated molecular weight 462 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC014224
Gene Symbol EEF1A1
Gene ID (NCBI) 1915
RRIDAB_3672493
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP68104
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell growth and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EEF1A1.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 EEF1A1 antibody CL488-11402Download protocol
FC protocol for CL Plus 488 EEF1A1 antibody CL488-11402Download protocol
Standard Protocols
Click here to view our Standard Protocols
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