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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated EEF1A2 Monoclonal an (CL488-66806)

    IF Staining of U2OS using CL488-66806

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 EEF1A2 antibody (CL488-66806, Clone: 4F3F4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated EEF1A2 Monoclonal an (CL488-66806)

    FC experiment of U2OS using CL488-66806

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated EEF1A2 Monoclonal antibody (CL488-66806, Clone:4F3F4)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated EEF1A2 Monoclonal antibody

EEF1A2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66806
Clone No.4F3F4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

Eukaryotic elongation factor 1 A-2, Elongation factor 1-alpha 2, EF-1-alpha-2, EEF1AL, eEF1A-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66806 targets EEF1A2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEF1A2 fusion protein Ag6875 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 alpha 2
Calculated molecular weight 50 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC000432
Gene Symbol EEF1A2
Gene ID (NCBI) 1917
RRIDAB_2934496
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ05639
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

eEF1A2 is one of two isoforms (eEF1A1 and eEF1A2) of eukaryotic elongation factor 1 alpha (eEF1A or eEF1α). The two isoforms share more than 90% sequence identity and have the similar function that being a protein translation factor involved in protein synthesis. Addition, eEF1A2 plays an important role in cell cycle regulation, heat-shock response, aging, posttranslational modifications, and signal transduction (PMID: 25905039). eEF1A2 is expressed in heart, brain tissue, diaphragm and skeletal muscle while eEF1A1 is expressed ubiquitously (PMID:14588074). And the eEF1A2 protein stimulates the phospholipid signaling and activates the Akt-dependent cell migration and actin remodeling that ultimately favors tumorigenesis. It is reported that eEF1A2 was related with kinds of cancers such as ovarian cancer, prostate cancer, pancreatic cancer, breast cancer and lung cancer (PMID:14588074; 24853801; 25744894; 25905039). An 48 kDa band has also been reported(PMID: 24285179).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 EEF1A2 antibody CL488-66806Download protocol
FC protocol for CL Plus 488 EEF1A2 antibody CL488-66806Download protocol
Standard Protocols
Click here to view our Standard Protocols