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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated EEF1B2 Monoclonal an (CL488-60329)

    IF Staining of HeLa using CL488-60329

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-60329 (EEF1B2 antibody) at dilution of 1:100.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated EEF1B2 Monoclonal antibody

EEF1B2 Monoclonal Antibody for IF/ICC
Cat No. CL488-60329
Clone No.3E1C11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

EF-1-beta, EF1B, EF 1 beta, EEF1B1, EEF1B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60329 targets EEF1B2 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEF1B2 fusion protein Ag0135 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 beta 2
Calculated molecular weight 25 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC000211
Gene Symbol EEF1B2
Gene ID (NCBI) 1933
RRIDAB_2883144
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP24534
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 EEF1B2 antibody CL488-60329Download protocol
Standard Protocols
Click here to view our Standard Protocols