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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EEF2 Polyclonal antibody (20107-1-AP)

    WB analysis using 20107-1-AP (same clone as 20107-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EEF2 Polyclonal antibody (20107-1-AP)

    WB analysis of HEK-293 using 20107-1-AP (same clone as 20107-1-PBS)

    WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HEK-293 cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EEF2 Polyclonal antibody (20107-1-AP)

    WB analysis of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HeLa cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using EEF2 Polyclonal antibody (20107-1-AP)

    WB analysis of NIH/3T3 using 20107-1-AP (same clone as 20107-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using EEF2 Polyclonal antibody (20107-1-AP)

    IP experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    IP result of anti-EEF2 (IP:20107-1-AP, 3ug; Detection:20107-1-AP 1:500) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EEF2 Polyclonal antibody (20107-1-AP)

    IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EEF2 Polyclonal antibody (20107-1-AP)

    IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using EEF2 Polyclonal antibody (20107-1-AP)

    IHC staining of human colon using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20107-1-AP (EEF2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EEF2 Polyclonal antibody (20107-1-AP)

    IHC staining of human breast cancer using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EEF2 Polyclonal antibody (20107-1-AP)

    IHC staining of human breast cancer using 20107-1-AP (same clone as 20107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EEF2 Polyclonal antibody (20107-1-AP)

    IF Staining of HepG2 using 20107-1-AP (same clone as 20107-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (20107-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using EEF2 Polyclonal antibody (20107-1-AP)

    FC experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human EEF2 (20107-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

EEF2 Polyclonal antibody, PBS Only

EEF2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 20107-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20107-1-PBS targets EEF2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF2 fusion protein Ag13826 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 2
Calculated molecular weight 858 aa, 95 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC126259
Gene Symbol EEF2
Gene ID (NCBI) 1938
RRIDAB_10950401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13639
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.