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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EEF2 Monoclonal antibody

EEF2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E4D5

Cat no : 67550-1-Ig

Synonyms

EEF 2, EEF2, EF 2, EF2, Elongation factor 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EEF2 Monoclonal antibody (67550-1-Ig)

    WB analysis using 67550-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EEF2 Monoclonal antibody (67550-1-Ig)

    WB analysis using 67550-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EEF2 Monoclonal antibody (67550-1-Ig)

    IHC staining of human breast cancer using 67550-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EEF2 Monoclonal antibody (67550-1-Ig)

    IHC staining of human colon cancer using 67550-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EEF2 Monoclonal antibody (67550-1-Ig)

    IHC staining of human colon cancer using 67550-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EEF2 Monoclonal antibody (67550-1-Ig)

    IHC staining of human breast cancer using 67550-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EEF2 Monoclonal antibody (67550-1-Ig)

    IF Staining of HepG2 using 67550-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67550-1-Ig (EEF2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67550-1-Ig targets EEF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEF2 fusion protein Ag13859 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 2
Calculated molecular weight 858 aa, 95 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC126259
Gene symbol EEF2
Gene ID (NCBI) 1938
RRIDAB_2882765
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EEF2 antibody 67550-1-IgDownload protocol
IHC protocol for EEF2 antibody 67550-1-IgDownload protocol
IF protocol for EEF2 antibody 67550-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols