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EEF2 Monoclonal antibody

EEF2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67550-1-Ig
Clone No.1E4D5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, LoVo cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, 4T1 cells, MCF-7 cells, pig brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67550-1-Ig targets EEF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEF2 fusion protein Ag13859 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 2
Calculated molecular weight 858 aa, 95 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC126259
Gene Symbol EEF2
Gene ID (NCBI) 1938
RRIDAB_2882765
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13639
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.

Protocols

Product Specific Protocols
WB protocol for EEF2 antibody 67550-1-IgDownload protocol
IHC protocol for EEF2 antibody 67550-1-IgDownload protocol
IF protocol for EEF2 antibody 67550-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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