Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HSC-T6 cells, LoVo cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, 4T1 cells, MCF-7 cells, pig brain tissue |
Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67550-1-Ig targets EEF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | EEF2 fusion protein Ag13859 相同性解析による交差性が予測される生物種 |
Full Name | eukaryotic translation elongation factor 2 |
Calculated molecular weight | 858 aa, 95 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | BC126259 |
Gene Symbol | EEF2 |
Gene ID (NCBI) | 1938 |
RRID | AB_2882765 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P13639 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.
Protocols
Product Specific Protocols | |
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WB protocol for EEF2 antibody 67550-1-Ig | Download protocol |
IHC protocol for EEF2 antibody 67550-1-Ig | Download protocol |
IF protocol for EEF2 antibody 67550-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |