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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EEF2 Monoclonal antibody, PBS Only

EEF2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E4D5

Cat no : 67550-1-PBS

Synonyms

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EEF2 Monoclonal antibody (67550-1-Ig)

    WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EEF2 Monoclonal antibody (67550-1-Ig)

    WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EEF2 Monoclonal antibody (67550-1-Ig)

    IHC staining of human breast cancer using 67550-1-Ig (same clone as 67550-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EEF2 Monoclonal antibody (67550-1-Ig)

    IHC staining of human colon cancer using 67550-1-Ig (same clone as 67550-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EEF2 Monoclonal antibody (67550-1-Ig)

    IHC staining of human colon cancer using 67550-1-Ig (same clone as 67550-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EEF2 Monoclonal antibody (67550-1-Ig)

    IHC staining of human breast cancer using 67550-1-Ig (same clone as 67550-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EEF2 Monoclonal antibody (67550-1-Ig)

    IF Staining of HepG2 using 67550-1-Ig (same clone as 67550-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (67550-1-Ig, Clone: 1E4D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EEF2 Monoclonal antibody (67550-1-Ig)

    IF Staining of HepG2 using 67550-1-Ig (same clone as 67550-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67550-1-Ig (EEF2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67550-1-PBS targets EEF2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEF2 fusion protein Ag13859 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 2
Calculated molecular weight 858 aa, 95 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC126259
Gene symbol EEF2
Gene ID (NCBI) 1938
RRIDAB_2882765
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.