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- KD/KO Validated
CRACR2A Polyclonal antibody
CRACR2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15206-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, Daudi cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IF | See 3 publications below |
| IP | See 2 publications below |
Product Information
15206-1-AP targets CRACR2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CRACR2A fusion protein Ag7358 相同性解析による交差性が予測される生物種 |
| Full Name | EF-hand calcium binding domain 4B |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 46 kDa, 80-90 kDa |
| GenBank accession number | BC004524 |
| Gene symbol | CRACR2A |
| Gene ID (NCBI) | 84766 |
| RRID | AB_2246413 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CRACR2A antibody 15206-1-AP | Download protocol |
| IHC protocol for CRACR2A antibody 15206-1-AP | Download protocol |
| IF protocol for CRACR2A antibody 15206-1-AP | Download protocol |
| IP protocol for CRACR2A antibody 15206-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder. | ||
Sci Rep Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells. | ||
Biochem Biophys Res Commun A long variant of CRACR2A that belongs to the Rab GTPase protein family.
| ||
J Cell Biol Rab46 integrates Ca2+ and histamine signaling to regulate selective cargo release from Weibel-Palade bodies.
| ||
