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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated CRACR2A Polyclonal a (CL488-15206)

    IF Staining of HEK-293 using CL488-15206

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 CRACR2A antibody (CL488-15206) at dilution of 1:200.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated CRACR2A Polyclonal antibody

CRACR2A Polyclonal Antibody for IF/ICC
Cat No. CL488-15206

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

CRAC channel regulator 2A, CRACR2A, DKFZp686G13246, EFCAB4B, FLJ33046, FLJ33805, Rab46

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15206 targets CRACR2A in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRACR2A fusion protein Ag7358 相同性解析による交差性が予測される生物種
Full Name EF-hand calcium binding domain 4B
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa, 80-90 kDa
GenBank accession numberBC004524
Gene Symbol CRACR2A
Gene ID (NCBI) 84766
RRIDAB_3672625
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSW2
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CRACR2A antibody CL488-15206Download protocol
Standard Protocols
Click here to view our Standard Protocols