Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 12004-1-AP (same clone as 12004-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.
WB analysis of mouse kidney using 12004-1-AP (same clone as 12004-1-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 12004-1-AP (same clone as 12004-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.
IP experiment of mouse kidney using 12004-1-AP (same clone as 12004-1-PBS)
IP result of anti-EFEMP2 (IP:12004-1-AP, 4ug; Detection:12004-1-AP 1:1000) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 12004-1-AP (same clone as 12004-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 12004-1-AP (same clone as 12004-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

EFEMP2 Polyclonal antibody, PBS Only

EFEMP2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12004-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12004-1-PBS targets EFEMP2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EFEMP2 fusion protein Ag2640 相同性解析による交差性が予測される生物種
Full Name	EGF-containing fibulin-like extracellular matrix protein 2
Calculated molecular weight	443 aa, 49 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC010456
Gene Symbol	EFEMP2
Gene ID (NCBI)	30008
RRID	AB_10667002
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95967
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

EFEMP2, also known as Fibulin-4 is a 60 kDa extracellular matrix (ECM) glycoprotein that is a pivotal oncogene in tumor progression and metastasis. EFEMP2 is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12004-1-AP (same clone as 12004-1-PBS)

WB analysis of mouse kidney using 12004-1-AP (same clone as 12004-1-PBS)

WB analysis of K-562 using 12004-1-AP (same clone as 12004-1-PBS)

IP experiment of mouse kidney using 12004-1-AP (same clone as 12004-1-PBS)

IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)

IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)

IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)

IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)

IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)

IHC staining of human prostate cancer using 12004-1-AP (same clone as 12004-1-PBS)

IHC staining of human prostate cancer using 12004-1-AP (same clone as 12004-1-PBS)

IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)

IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)

IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)

EFEMP2 Polyclonal antibody, PBS Only

EFEMP2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information