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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EFEMP2 Polyclonal antibody (12004-1-AP)

    WB analysis using 12004-1-AP (same clone as 12004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    WB analysis of mouse kidney using 12004-1-AP (same clone as 12004-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using EFEMP2 Polyclonal antibody (12004-1-AP)

    WB analysis of K-562 using 12004-1-AP (same clone as 12004-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IP experiment of mouse kidney using 12004-1-AP (same clone as 12004-1-PBS)

    IP result of anti-EFEMP2 (IP:12004-1-AP, 4ug; Detection:12004-1-AP 1:1000) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human prostate cancer using 12004-1-AP (same clone as 12004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human prostate cancer using 12004-1-AP (same clone as 12004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human heart using 12004-1-AP (same clone as 12004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using EFEMP2 Polyclonal antibody (12004-1-AP)

    IHC staining of human skin using 12004-1-AP (same clone as 12004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12004-1-PBS in a different storage buffer formulation.

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EFEMP2 Polyclonal antibody, PBS Only

EFEMP2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12004-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12004-1-PBS targets EFEMP2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EFEMP2 fusion protein Ag2640 相同性解析による交差性が予測される生物種
Full Name EGF-containing fibulin-like extracellular matrix protein 2
Calculated molecular weight 443 aa, 49 kDa
Observed molecular weight60 kDa
GenBank accession numberBC010456
Gene Symbol EFEMP2
Gene ID (NCBI) 30008
RRIDAB_10667002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95967
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EFEMP2, also known as Fibulin-4 is a 60 kDa extracellular matrix (ECM) glycoprotein that is a pivotal oncogene in tumor progression and metastasis. EFEMP2 is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome.