Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-83264-5 targets EFHD2 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | EFHD2 fusion protein Ag35084 相同性解析による交差性が予測される生物種 |
| Full Name | EF-hand domain family, member D2 |
| Calculated molecular weight | 27 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC023611 |
| Gene Symbol | EFHD2 |
| Gene ID (NCBI) | 79180 |
| RRID | AB_3673230 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96C19 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EFHD2 also named Swiprosin-1, is a Ca2+ binding actin protein. The expression of EFHD2 is upregulated during the activation of immune cells, epithelial and endothelial cells. The expression of EFHD2 is regulated by diverse signaling pathways that are contingent upon the specific type of cells. EFHD2 consists of 240 amino-acids. Based on the composition, the predicted molecular weight was reported to be 27 KDa while the apparent molecular weight was 33 KDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 EFHD2 antibody CL488-83264-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |