Pikachurin Monoclonal antibody

Pikachurin Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C6D12

Cat no : 66232-1-Ig

Synonyms

Agrin like protein, AGRINL, AGRNL, EGFLAM, Pikachurin


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Tested Applications

Positive WB detected inhuman retina tissue, PC-3 cells
Positive IF/ICC detected inY79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

66232-1-Ig targets Pikachurin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Pikachurin fusion protein Ag6362 相同性解析による交差性が予測される生物種
Full Name EGF-like, fibronectin type III and laminin G domains
Calculated molecular weight 111 kDa
Observed molecular weight 111 kDa, 55-60 kDa
GenBank accession numberBC033177
Gene symbol Pikachurin
Gene ID (NCBI) 133584
RRIDAB_2881623
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).

Protocols

Product Specific Protocols
WB protocol for Pikachurin antibody 66232-1-IgDownload protocol
IF protocol for Pikachurin antibody 66232-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int Immunopharmacol

EGFLAM exhibits oncogenic activity and shows promise as a prognostic biomarker and therapeutic target in glioblastoma

Authors - Kaishu Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Federico (Verified Customer) (02-18-2020)

The antibody works extremely well in western blot analyses using both recombinant protein fragments produced in HEK293 cells as well as the endogenous protein from Y79 medium. Very sensitive and specific.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 2000x
  • Cell Tissue Type: overexpressing protein samples from HEK293 cells, conditioned media from Y79 cells