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Validation Data Gallery

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  • Western Blot (WB) analysis of human retina tissue using Pikachurin Monoclonal antibody (66232-1-Ig)

    WB analysis of human retina using 66232-1-Ig

    human retina tissue were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using Pikachurin Monoclonal antibody (66232-1-Ig)

    WB analysis of PC-3 using 66232-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of rat eye tissue using Pikachurin Monoclonal antibody (66232-1-Ig)

    IF Staining of rat eye using 66232-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Y79 cells using Pikachurin Monoclonal antibody (66232-1-Ig)

    IF Staining of Y79 using 66232-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Pikachurin Monoclonal antibody

Pikachurin Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA
Cat No. 66232-1-Ig
Clone No.1C6D12
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

EGFLAM, AGRNL, Agrin-like protein, AGRINL, Agrin like protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman retina tissue, PC-3 cells
Positive IF-P detected inrat eye tissue
Positive IF/ICC detected inY79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

66232-1-Ig targets Pikachurin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Pikachurin fusion protein Ag6362 相同性解析による交差性が予測される生物種
Full Name EGF-like, fibronectin type III and laminin G domains
Calculated molecular weight 111 kDa
Observed molecular weight 111 kDa, 55-60 kDa
GenBank accession numberBC033177
Gene Symbol Pikachurin
Gene ID (NCBI) 133584
RRIDAB_2881623
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ63HQ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).

Protocols

Product Specific Protocols
WB protocol for Pikachurin antibody 66232-1-IgDownload protocol
IF protocol for Pikachurin antibody 66232-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int Immunopharmacol

EGFLAM exhibits oncogenic activity and shows promise as a prognostic biomarker and therapeutic target in glioblastoma

Authors - Kaishu Li
View Article