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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Pikachurin Monoclonal antibody, PBS Only

Pikachurin Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C6D12

Cat no : 66232-1-PBS

Synonyms

Agrin like protein, AGRINL, AGRNL, EGFLAM, Pikachurin

Validation Data Gallery

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  • Western Blot (WB) analysis of human retina tissue using Pikachurin Monoclonal antibody (66232-1-Ig)

    WB analysis of human retina using 66232-1-Ig (same clone as 66232-1-PBS)

    human retina tissue were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using Pikachurin Monoclonal antibody (66232-1-Ig)

    WB analysis of PC-3 using 66232-1-Ig (same clone as 66232-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Y79 cells using Pikachurin Monoclonal antibody (66232-1-Ig)

    IF Staining of Y79 using 66232-1-Ig (same clone as 66232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66232-1-PBS targets Pikachurin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Pikachurin fusion protein Ag6362 相同性解析による交差性が予測される生物種
Full Name EGF-like, fibronectin type III and laminin G domains
Calculated molecular weight 111 kDa
Observed molecular weight 111 kDa, 55-60 kDa
GenBank accession numberBC033177
Gene symbol Pikachurin
Gene ID (NCBI) 133584
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).