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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EGLN3/PHD3 Recombinant antibody, PBS Only

EGLN3/PHD3 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1O19

Cat no : 82377-1-PBS

Synonyms

Egl nine homolog 3, EGLN3, FLJ21620, HIF PH3, HIF prolyl hydroxylase 3, HIFPH3, HPH 1, HPH 3, PHD3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EGLN3/PHD3 Recombinant antibody (82377-1-RR)

    WB analysis using 82377-1-RR (same clone as 82377-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82377-1-RR (EGLN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82377-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EGLN3/PHD3 Recombinant antibody (82377-1-RR)

    IF Staining of HepG2 using 82377-1-RR (same clone as 82377-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EGLN3/PHD3 antibody (82377-1-RR, Clone: 1O19 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82377-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using EGLN3/PHD3 Recombinant antibody (82377-1-RR)

    IF Staining of U2OS using 82377-1-RR (same clone as 82377-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using EGLN3/PHD3 antibody (82377-1-RR, Clone: 1O19 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82377-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82377-1-PBS targets EGLN3/PHD3 in WB, IF, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EGLN3/PHD3 fusion protein Ag13197 相同性解析による交差性が予測される生物種
Full Name egl nine homolog 3 (C. elegans)
Calculated molecular weight 27 kDa
Observed molecular weight27-30 kDa
GenBank accession numberBC010992
Gene symbol EGLN3
Gene ID (NCBI) 112399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.