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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EGLN3/PHD3 Recombinant antibody (82377-1-RR)

    WB analysis using 82377-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82377-1-RR (EGLN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EGLN3/PHD3 Recombinant antibody (82377-1-RR)

    IF Staining of HepG2 using 82377-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EGLN3/PHD3 antibody (82377-1-RR, Clone: 1O19 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using EGLN3/PHD3 Recombinant antibody (82377-1-RR)

    IF Staining of U2OS using 82377-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using EGLN3/PHD3 antibody (82377-1-RR, Clone: 1O19 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of U2OS cells using EGLN3/PHD3 Recombinant antibody (82377-1-RR)

    FC experiment of U2OS using 82377-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug EGLN3/PHD3 Recombinant antibody (82377-1-RR, Clone:1O19) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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EGLN3/PHD3 Recombinant antibody

EGLN3/PHD3 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 82377-1-RR
Clone No.1O19

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

EGLN3, PHD3, HIF PH3, Egl nine homolog 3, EC:1.14.11.29

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue, rat kidney tissue
Positive IF/ICC detected inHepG2 cells, U2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82377-1-RR targets EGLN3/PHD3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EGLN3/PHD3 fusion protein Ag13197 相同性解析による交差性が予測される生物種
Full Name egl nine homolog 3 (C. elegans)
Calculated molecular weight 27 kDa
Observed molecular weight27-30 kDa
GenBank accession numberBC010992
Gene Symbol EGLN3
Gene ID (NCBI) 112399
RRIDAB_3670522
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H6Z9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

Protocols

Product Specific Protocols
WB protocol for EGLN3/PHD3 antibody 82377-1-RRDownload protocol
IF protocol for EGLN3/PHD3 antibody 82377-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols