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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EHD2 Polyclonal antibody (11440-1-AP)

    WB analysis using 11440-1-AP (same clone as 11440-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using EHD2 Polyclonal antibody (11440-1-AP)

    WB analysis of human heart using 11440-1-AP (same clone as 11440-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IP experiment of mouse heart using 11440-1-AP (same clone as 11440-1-PBS)

    IP result of anti-EHD2 (IP:11440-1-AP, 4ug; Detection:11440-1-AP 1:800) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human breast cancer using 11440-1-AP (same clone as 11440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human breast cancer using 11440-1-AP (same clone as 11440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human colon using 11440-1-AP (same clone as 11440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11440-1-AP (EHD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human colon using 11440-1-AP (same clone as 11440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11440-1-AP (EHD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human oesophagus cancer using 11440-1-AP (same clone as 11440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human oesophagus cancer using 11440-1-AP (same clone as 11440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human ovary tumor using 11440-1-AP (same clone as 11440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EHD2 Polyclonal antibody (11440-1-AP)

    IHC staining of human ovary tumor using 11440-1-AP (same clone as 11440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

EHD2 Polyclonal antibody, PBS Only

EHD2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11440-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

PAST2, PAST homolog 2, EH domain-containing protein 2, EH domain containing protein 2, EH domain containing 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11440-1-PBS targets EHD2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EHD2 fusion protein Ag2002 相同性解析による交差性が予測される生物種
Full Name EH-domain containing 2
Calculated molecular weight 543 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC014445
Gene Symbol EHD2
Gene ID (NCBI) 30846
RRIDAB_2097325
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZN4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EH domain-containing protein 2 (EHD2) is a member of the EHD protein family. It is primarily located in the plasma membrane but can also be found in the cytoplasm and endosomes. EHD2 is also a nuclear-cytoplasmic shuttle protein, acting as a corepressor of transcription to inhibit gene transcription when it enters the cell nucleus. EHD2 is a dynamin-related ATPase that plays a key role in regulating a series of biological processes. As a crucial regulator of endocytic transport, it is involved in the formation and maintenance of endosomal tubules and vesicles, which are vital for intracellular transport of proteins and other substances. EHD2 has been implicated in several human cancers and has potential as a predictive biomarker of chemotherapy efficacy.