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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EI24 Polyclonal antibody (20456-1-AP)

    WB analysis using 20456-1-AP (same clone as 20456-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20456-1-AP (EI24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20456-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EI24 Polyclonal antibody (20456-1-AP)

    IHC staining of human colon cancer using 20456-1-AP (same clone as 20456-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20456-1-AP (EI24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20456-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EI24 Polyclonal antibody (20456-1-AP)

    IHC staining of human colon cancer using 20456-1-AP (same clone as 20456-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20456-1-AP (EI24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20456-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

EI24 Polyclonal antibody, PBS Only

EI24 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20456-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20456-1-PBS targets EI24 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EI24 fusion protein Ag14169 相同性解析による交差性が予測される生物種
Full Name etoposide induced 2.4 mRNA
Calculated molecular weight 340 aa, 39 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC002390
Gene Symbol EI24
Gene ID (NCBI) 9538
RRIDAB_10858326
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14681
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ei24 is an ER-localized protein originally identified as a p53-induced proapoptotic gene in etoposide-treated NIH3T3 cells. It has been shown to be able to bind to the antiapoptotic protein Bcl-2 (PMID: 15781622), play a role in autophagy (PMID: 23074225), and induce proliferate arrest/apoptosis (PMID: 10594026). Ei24 can bind specifically to IMPβ1 and IMPα2 to impede their normal role in nuclear import (PMID: 24821838).