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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF1 Monoclonal antibody

EIF1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G8C6

Cat no : 67601-1-Ig

Synonyms

A121, EIF 1, EIF1, EIF1A, ISO1, SUI1, Sui1iso1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using EIF1 Monoclonal antibody (67601-1-Ig)

    WB analysis of HEK-293 using 67601-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67601-1-Ig (EIF1 antibody) at dilution of 1:3200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using EIF1 Monoclonal antibody (67601-1-Ig)

    WB analysis of Jurkat using 67601-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67601-1-Ig (EIF1 antibody) at dilution of 1:3200 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF1 Monoclonal antibody (67601-1-Ig)

    IF Staining of HepG2 using 67601-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67601-1-Ig (EIF1 antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF1 Monoclonal antibody (67601-1-Ig)

    IF Staining of HepG2 using 67601-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF1 antibody (67601-1-Ig, Clone: 2G8C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6400
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67601-1-Ig targets EIF1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EIF1 fusion protein Ag7843 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 1
Calculated molecular weight 13 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC005118
Gene symbol EIF1
Gene ID (NCBI) 10209
RRIDAB_2882807
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

It is a necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Molecular weight is 13kd.

Protocols

Product Specific Protocols
WB protocol for EIF1 antibody 67601-1-IgDownload protocol
IF protocol for EIF1 antibody 67601-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols