Validation Data Gallery
Filter:
Tested Applications
| Positive IF/ICC detected in | MCF-7 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-67601 targets EIF1 in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | EIF1 fusion protein Ag7843 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic translation initiation factor 1 |
| Calculated molecular weight | 13 kDa |
| Observed molecular weight | 13 kDa |
| GenBank accession number | BC005118 |
| Gene Symbol | EIF1 |
| Gene ID (NCBI) | 10209 |
| RRID | AB_2920163 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P41567 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
It is a necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Molecular weight is 13kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 EIF1 antibody CL594-67601 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |