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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated EIF1 Monoclonal antibody

EIF1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G8C6

Cat no : CL594-67601

Synonyms

A121, EIF 1, EIF1, EIF1A, ISO1, SUI1, Sui1iso1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated EIF1 Monoclonal antibody (CL594-67601)

    IF Staining of HepG2 using CL594-67601

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 EIF1 antibody (CL594-67601, Clone: 2G8C6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated EIF1 Monoclonal antibody (CL594-67601)

    IF Staining of MCF-7 using CL594-67601

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 EIF1 antibody (CL594-67601, Clone: 2G8C6 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inMCF-7 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67601 targets EIF1 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EIF1 fusion protein Ag7843 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 1
Calculated molecular weight 13 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC005118
Gene symbol EIF1
Gene ID (NCBI) 10209
RRIDAB_2920163
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

It is a necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Molecular weight is 13kd.

Protocols

Product Specific Protocols
IF protocol for CL594 EIF1 antibody CL594-67601Download protocol
Standard Protocols
Click here to view our Standard Protocols